Definitive diagnosis rests on isolation of the virus by means of tissue culture or RT-PCR assay. However, isolation of Ebola virus in tissue culture is a high-risk procedure that can be performed safely only in a few high-containment laboratories throughout the world.
For more on the work-up of patients with Ebola virus, read here.
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Cite this: Fast Five Quiz: How Much Do You Really Know About Ebola Virus? - Medscape - Sep 22, 2014.